A reference chromosomal map of the hot chili pepper Capsicum pubescens cv. “locoto” (Solanaceae)

Abstract Capsicum pubescens is a cultivated hot chili pepper, consumed in Latin American cuisine as distinctive ingredient, and popularly known “locoto” or “rocoto”. This taxon also an outstanding source of resistance to biotic abiotic stresses well other valuable fruit traits for breeding the worldwidely C. annuum related species. In this study, chromosome complement cv. (2n = 24) was deeply characterized through sequential combination conventional molecular cytogenetics approaches comprising: Ag-NOR staining, heterochromatic fluorescent C-DAPI, DAPI/AMD-CMA/DA bandings, fluorescence situ hybridization (FISH) Capsicum-derived probes 5S 18S-25S rRNA genes different regions spacers ribosomal unit, telomeric probe. The markers identified were systematically combined with morphological karyotype parameters - number, size, centromeres, satellites produce physical map which allowed identification several landmarks each individual chromosome. reference chromosomal here presented most comprehensively developed so far. It envisioned that will serve framework upcoming sequencing projects starting point assist future genetic mapping important agronomic traits.